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celltrace far red cell proliferation kit  (Thermo Fisher)


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    Thermo Fisher celltrace far red cell proliferation kit
    Celltrace Far Red Cell Proliferation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/celltrace+far+red+cell+proliferation+kit/celltrace+violet/pm40615696-257-7-13
    Average 90 stars, based on 1 article reviews
    celltrace far red cell proliferation kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Labeling:

    Article Title: HIV-1 Gag-protease-driven replicative capacity influences T-cell metabolism, cytokine induction, and viral cell-to-cell spread
    Article Snippet: These were designated as infected donor cells and labeled with cell proliferation eFlour450 dye (ThermoFisher Scientific, Massachusetts). .. Fresh Jurkat cells, designated as target cells were labeled using the CellTrace Far red cell proliferation kit (ThermoFisher Scientific, USA) and co-cultured 1:1 with infected donor cells for up to 48 hours (0.5 × 10 6 cells in total). .. HIV-1 cell-to-cell spread was measured after 24 and 48 hours by Gag p24 detection within target cells using an LSRFortessa (BD Biosciences) with FACSDiva software.

    Article Title: CD2 augmentation enhances CAR-T-cell efficacy via immunological synapse remodeling and T-cell exhaustion mitigation.
    Article Snippet: .. Jurkat CAR-T cells were labeled with the CellTrace Far Red Cell Proliferation Kit (Thermo Fisher Scientific) prior to the experiments. ..

    Article Title: HIV-1 Gag-protease-driven replicative capacity influences T-cell metabolism, cytokine induction, and viral cell-to-cell spread.
    Article Snippet: These were designated as infected donor cells and labeled with cell proliferation eFlour450 dye (ThermoFisher Scientific, Massachusetts). .. Fresh Jurkat cells, designated as target cells were labeled using the CellTrace Far red cell proliferation kit (ThermoFisher Scientific, USA) and co-cultured 1:1 with infected donor cells for up to 48 hours (0.5 × 106 cells in total). .. HIV-1 cell-to-cell spread was measured after 24 and 48 hours by Gag p24 detection within target cells using an LSRFortessa (BD Biosciences) with FACSDiva software.

    Infection:

    Article Title: HIV-1 Gag-protease-driven replicative capacity influences T-cell metabolism, cytokine induction, and viral cell-to-cell spread
    Article Snippet: These were designated as infected donor cells and labeled with cell proliferation eFlour450 dye (ThermoFisher Scientific, Massachusetts). .. Fresh Jurkat cells, designated as target cells were labeled using the CellTrace Far red cell proliferation kit (ThermoFisher Scientific, USA) and co-cultured 1:1 with infected donor cells for up to 48 hours (0.5 × 10 6 cells in total). .. HIV-1 cell-to-cell spread was measured after 24 and 48 hours by Gag p24 detection within target cells using an LSRFortessa (BD Biosciences) with FACSDiva software.

    Article Title: HIV-1 Gag-protease-driven replicative capacity influences T-cell metabolism, cytokine induction, and viral cell-to-cell spread.
    Article Snippet: These were designated as infected donor cells and labeled with cell proliferation eFlour450 dye (ThermoFisher Scientific, Massachusetts). .. Fresh Jurkat cells, designated as target cells were labeled using the CellTrace Far red cell proliferation kit (ThermoFisher Scientific, USA) and co-cultured 1:1 with infected donor cells for up to 48 hours (0.5 × 106 cells in total). .. HIV-1 cell-to-cell spread was measured after 24 and 48 hours by Gag p24 detection within target cells using an LSRFortessa (BD Biosciences) with FACSDiva software.

    Recombinant:

    Article Title: High-resolution dynamic imaging of chromatin DNA communication using Oligo-LiveFISH.
    Article Snippet: In brief Oligo-LiveFISH is an approach that can track diverse genomic loci at high spatial (20 nm) and temporal (50 ms) resolution and can work in diverse cell types, including primary cells.. Combined with super-localization microscopy and dynamic modeling, Oligo-LiveFISH reveals distinct modes of chromatin communication and the dynamic relationship between transcription and enhancer-promoter interactions.

    Fluorescence In Situ Hybridization:

    Article Title: High-resolution dynamic imaging of chromatin DNA communication using Oligo-LiveFISH.
    Article Snippet: In brief Oligo-LiveFISH is an approach that can track diverse genomic loci at high spatial (20 nm) and temporal (50 ms) resolution and can work in diverse cell types, including primary cells.. Combined with super-localization microscopy and dynamic modeling, Oligo-LiveFISH reveals distinct modes of chromatin communication and the dynamic relationship between transcription and enhancer-promoter interactions.

    Hybridization:

    Article Title: High-resolution dynamic imaging of chromatin DNA communication using Oligo-LiveFISH.
    Article Snippet: In brief Oligo-LiveFISH is an approach that can track diverse genomic loci at high spatial (20 nm) and temporal (50 ms) resolution and can work in diverse cell types, including primary cells.. Combined with super-localization microscopy and dynamic modeling, Oligo-LiveFISH reveals distinct modes of chromatin communication and the dynamic relationship between transcription and enhancer-promoter interactions.

    cDNA Synthesis:

    Article Title: High-resolution dynamic imaging of chromatin DNA communication using Oligo-LiveFISH.
    Article Snippet: In brief Oligo-LiveFISH is an approach that can track diverse genomic loci at high spatial (20 nm) and temporal (50 ms) resolution and can work in diverse cell types, including primary cells.. Combined with super-localization microscopy and dynamic modeling, Oligo-LiveFISH reveals distinct modes of chromatin communication and the dynamic relationship between transcription and enhancer-promoter interactions.

    SYBR Green Assay:

    Article Title: High-resolution dynamic imaging of chromatin DNA communication using Oligo-LiveFISH.
    Article Snippet: In brief Oligo-LiveFISH is an approach that can track diverse genomic loci at high spatial (20 nm) and temporal (50 ms) resolution and can work in diverse cell types, including primary cells.. Combined with super-localization microscopy and dynamic modeling, Oligo-LiveFISH reveals distinct modes of chromatin communication and the dynamic relationship between transcription and enhancer-promoter interactions.

    Gel Extraction:

    Article Title: High-resolution dynamic imaging of chromatin DNA communication using Oligo-LiveFISH.
    Article Snippet: In brief Oligo-LiveFISH is an approach that can track diverse genomic loci at high spatial (20 nm) and temporal (50 ms) resolution and can work in diverse cell types, including primary cells.. Combined with super-localization microscopy and dynamic modeling, Oligo-LiveFISH reveals distinct modes of chromatin communication and the dynamic relationship between transcription and enhancer-promoter interactions.

    Transfection:

    Article Title: High-resolution dynamic imaging of chromatin DNA communication using Oligo-LiveFISH.
    Article Snippet: In brief Oligo-LiveFISH is an approach that can track diverse genomic loci at high spatial (20 nm) and temporal (50 ms) resolution and can work in diverse cell types, including primary cells.. Combined with super-localization microscopy and dynamic modeling, Oligo-LiveFISH reveals distinct modes of chromatin communication and the dynamic relationship between transcription and enhancer-promoter interactions.

    Staining:

    Article Title: High-resolution dynamic imaging of chromatin DNA communication using Oligo-LiveFISH.
    Article Snippet: In brief Oligo-LiveFISH is an approach that can track diverse genomic loci at high spatial (20 nm) and temporal (50 ms) resolution and can work in diverse cell types, including primary cells.. Combined with super-localization microscopy and dynamic modeling, Oligo-LiveFISH reveals distinct modes of chromatin communication and the dynamic relationship between transcription and enhancer-promoter interactions.

    Article Title: Memory-like Natural Killer Cell and CD19 Antibody–Based Immunotherapy in Combination with Tyrosine Kinase Inhibition Has Antitumor Effects against Ph(-like) Acute Lymphoblastic Leukemia
    Article Snippet: Briefly, PBNKC were resuspended in culture media containing CellEvent caspase 3/7 green detection reagent (Thermo Fisher Scientific) and split into six different batches of PBNKC each treated with 0.1% DMSO, 1 μmol/L ruxolitinib, or 1 μmol/L imatinib. .. Target cells were counted and stained with CellTrace Far Red Cell Proliferation Kit (Thermo Fisher Scientific) according to manufacturer’s instructions (Supplementary Table S1). ..

    Article Title: CD2 augmentation enhances CAR-T-cell efficacy via immunological synapse remodeling and T-cell exhaustion mitigation.
    Article Snippet: .. Raji cells, which were stained with the CellTrace Far Red Cell Proliferation Kit (Thermo Fisher Scientific), were then added and cocultured with the stained CAR-T cells at a target ratio of 1:10 for 6 h. During this incubation period, samples of the cell suspension were collected every 2 h for flow cytometric analysis. ..

    Article Title: Targeting prostate cancer by new bispecific monocyte engager directed to prostate-specific membrane antigen.
    Article Snippet: Following polarization, the macrophages were stained in-plate with CellTrace CFSE Cell Proliferation Kit (Thermo Fisher Scientific). .. The tumor cells were harvested by trypsinization and stained with CellTrace Far Red Cell Proliferation Kit (Thermo Fisher Scientific) following the manufacturer’s instructions. ..

    Cell Isolation:

    Article Title: High-resolution dynamic imaging of chromatin DNA communication using Oligo-LiveFISH.
    Article Snippet: In brief Oligo-LiveFISH is an approach that can track diverse genomic loci at high spatial (20 nm) and temporal (50 ms) resolution and can work in diverse cell types, including primary cells.. Combined with super-localization microscopy and dynamic modeling, Oligo-LiveFISH reveals distinct modes of chromatin communication and the dynamic relationship between transcription and enhancer-promoter interactions.

    Hi-C:

    Article Title: High-resolution dynamic imaging of chromatin DNA communication using Oligo-LiveFISH.
    Article Snippet: In brief Oligo-LiveFISH is an approach that can track diverse genomic loci at high spatial (20 nm) and temporal (50 ms) resolution and can work in diverse cell types, including primary cells.. Combined with super-localization microscopy and dynamic modeling, Oligo-LiveFISH reveals distinct modes of chromatin communication and the dynamic relationship between transcription and enhancer-promoter interactions.

    Incubation:

    Article Title: CD2 augmentation enhances CAR-T-cell efficacy via immunological synapse remodeling and T-cell exhaustion mitigation.
    Article Snippet: .. Raji cells, which were stained with the CellTrace Far Red Cell Proliferation Kit (Thermo Fisher Scientific), were then added and cocultured with the stained CAR-T cells at a target ratio of 1:10 for 6 h. During this incubation period, samples of the cell suspension were collected every 2 h for flow cytometric analysis. ..

    Suspension:

    Article Title: CD2 augmentation enhances CAR-T-cell efficacy via immunological synapse remodeling and T-cell exhaustion mitigation.
    Article Snippet: .. Raji cells, which were stained with the CellTrace Far Red Cell Proliferation Kit (Thermo Fisher Scientific), were then added and cocultured with the stained CAR-T cells at a target ratio of 1:10 for 6 h. During this incubation period, samples of the cell suspension were collected every 2 h for flow cytometric analysis. ..

    Sequencing:

    Article Title: Gene editing without ex vivo culture evades genotoxicity in human hematopoietic stem cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rhesus CD34+ antibody (Clone 12.8) Fred Hutchinson Cancer Research Center N/A CD34-PE antibody (Clone 563) BD Biosciences # 550761 RRID:AB_393871 Mouse anti-human fetal hemoglobin BD Biosciences # 551796 RRID:AB_394253 APC rat anti-mouse IgG1 (Clone X56) BD Biosciences # 550874 RRID:AB_398470 CD14-FITC (Clone M5E2) BD Bioscience # 555397 RRID:AB_395798 CD20-PE (Clone 2H7) BD Bioscience # 555623 RRID:AB_395989 CD34-APC (Clone 563) BD Bioscience # 561209 RRID:AB_10683161 CD45-V450 (Clone D058-1283) BD Bioscience # 561291 RRID:AB_10611576 CD71-PE (Clone DF1513) GeneTex # GTX43030 RRID:AB_11170434 CD45-FITC (Clone MB4-6D6) Miltenyi Biotech # 130-119-764 RRID:AB_2751825 CD34-BV785 (Clone 561) BioLegend # 343626 RRID:AB_2632727 CD90-APC (Clone 5E10) BioLegend # 328114 RRID:AB_893440 CD45RA-VioBlue (Clone T6D11) Miltenyi Biotech # 130-113-360 RRID:AB_2726132 CD117-PE (Clone 104D2) BioLegend # 313204 RRID:AB_314983 Goat anti-human IgG-AF488 Jackson ImmunoResearch # 109-545-088 RRID:AB_2337838 CD45-APC (Non-competing MB4-6D6 clone) Miltenyi Biotech # 130-123-787 RRID:AB_2819522 CD3-BV605 (Clone SP34) BD Bioscience # 562994 RRID:AB_2737938 CD8-APC-Vio 770 (Clone BW135/80) Miltenyi Biotech # 130-113-155 RRID:AB_2725983 CD4-BV650 (Clone L200) BD Bioscience # 563737 RRID:AB_2687486 CD20-BV421 (Clone 2H7) BioLegend # 302330 RRID:AB_10965543 CD45RA-PerCP-Cy5.5 (Clone T6D11) Miltenyi Biotech # 130-113-359 RRID:AB_2751109 CD34-BV785 (Clone 561) BioLegend # 343626 RRID:AB_2632727 CD90-APC (Clone 5E10) BioLegend # 328114 RRID:AB_893440 Anti-mouse IgM beads Miltenyi Biotech # 130-047-302 RRID:AB_244359 Biological samples Frozen healthy human umbilical cord derived CD34+ HSPCs AllCells N/A Frozen/fresh rhesus CD34+ HSPCs NIH N/A Cryopreserved bone marrow CD34+ HSPCs StemCell Technologies # 70002 Chemicals, peptides, and recombinant proteins 7-AAD BioLegend # 420403 Granulocyte-Colony Stimulating Factor (G-CSF) Amgen NDC 55513-0530-10 Plerixafor Sigma-Aldrich # 110078-46-1 Ficoll-Paque PLUS density gradient media GE Healthcare # 17144002 Human stem cell factor (SCF) R&D Systems # 255-SC/CF Human thrombopoietin (TPO) R&D Systems # 288-TP/CF Human recombinant human Flt3-ligand, (Flt3) R&D Systems # 308-TP/CF 33NLS SpCas9 Laboratory of Scot A. Wolfe N/A Interleukin-3 (IL-3) R&D Systems 203-IL-100/CF (Continued on next page) Cell Stem Cell 32, 209–226.e1–e8, February 6, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Erythropoietin (EPO) Amgen # 55513-144-10 Dexamethasone VETone # 501012 Depo-estradiol Pfizer NDC 00009-0271-01 Fetal bovine serum (FBS) ThermoFisher Scientific # 43803 FBS Gemini # 100-106 Insulin Lily NDC 00002-8215 Holo-transferrin Sigma Aldrich # T0665-1G Bovine serum albumin (BSA) Roche # 3116956001 TCEP Thermo Fisher Scientific # 20490 Hoechst 33342 Thermo Fisher Scientific # R37165 IL-6 R&D Systems # 206-IL-200/CF IL-2 NCI # Ro 23-6019 RetroNectin (Takara Bio) # T100B Busulfex PDL BioPharma NDC 59148-070-90 Hydroxyurea Bristol-Myers Squibb NDC 68084- 284-01 Critical commercial assays Gibco CTS Detachable Dynabeads CD3/CD28 Kit Thermo Fisher Scientific # A56992 CellTrace Far Red Cell Proliferation Kit Thermo Fisher Scientific # C34564 Deposited data Raw sequencing data This paper PRJNA1045609 Experimental models: Cell lines .. Experimental models: Organisms/strains Mouse: NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NGS) The Jackson Laboratory JAX: 005557 Oligonucleotides sgRNAs This paper Table S3 Primers for amplicon sequencing This paper Table S3 Primers and probes for ddPCR This paper Table S3 Software and algorithms FlowJo N/A http://www.flowjo.com CRISPResso2 N/A http://crispresso2.pinellolab.org Centara Phoenix WinNonLin software N/A https://www.certara.com/ software/phoenix-pkpd/ CRIS.py N/A https://github.com/patrickc01/CRIS.py GraphPad Prism 9 software N/A https://www.graphpad.com/



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